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KMID : 1025520070490010015
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Journal of Animal Science and Technology
2007 Volume.49 No. 1 p.15 ~ p.24
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Expression Profiling of Connexins in the Efferent Ductules of Male Rats During Postnatal Development
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Lee Ki-Ho
Choi In-Ho
Chung Chung-Soo
Chang J.-S.
Kim J. Y
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Abstract
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The purpose of this study was to evaluate the presence and differential expression of connexin isoforms in the efferent ductules (ED) of male rat reproductive tract during postnatal development. Total RNA was isolated from the ED collected from rats at 1 week, 2 weeks, 1 month, 3 months, and 6 months of ages. Expression of six connexin mRNAs of 14 isoforms tested was detected by semi-quantitative RT-PCR. Fluctuation of mRNA levels of connexins 26 and 30 was found according to ages. A significant decrease of connexin 31.1 mRNA level was observed in the ED at 1 month of age. The highest levels of connexin 37 and 45 mRNAs were detected in the ED of early developmental period, while the expression of connexin 43 was the lowest at 1 week of age. The present study demonstrates differential regulation on expression of connexin isoforms in the rat ED in age-dependent manner.
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KEYWORD
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Efferent ductules, Connexin, Male fertility, mRNA expression, Gap junction
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